Table of Contents |
Principle of gas chromatography (GC):
Applications of gas chromatography (GC):
Applications of gas liquid chromatography:
Commonly asked questions on chromatography are as follows.
Which
factors influence the separation of the components in gas chromatography?
The
separation of the components in GC is affected by the vapor pressure, length of
the column, the polarity of analyte versus the polarity of stationary phase on
the column, the temperature of column and oven, the flow rate of carrier gas
and amount of sample injected.
What
is the most commonly used detector in gas chromatography?
There
are many types of detectors are used in gas chromatography such as flame
ionization detector (FID), thermal conductivity detector (TCD), mass
spectrometer (GC/MS), thermal conductivity detector (TCD)and electron capture
detector (ECD), but out of these, the flame ionization detector is most
commonly used detector in gas chromatography.
What
is the major difference between GC-MS and LC-MS?
Both
GC-MS and LC-MS are separation technique used to separate the compounds. The
major difference between GC-MS and LC-MS is that the in GC-MS mobile phase uses
as inert gases such as nitrogen or helium, whereas LC-MS uses a solvent or
mixture of solvents as its mobile phase.
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