Saturday, May 2, 2020

Principle and applications of HPTLC

HPTLC is an advanced and automated version of thin layer chromatography with superior and higher separation efficiency and detection limits. It is also known as planar chromatography or high-pressure thin layer chromatography. It is a potent analytical technique and suitable for quantitative and qualitative analysis of components. In this type of chromatography, separation can result from the partition or, adsorption, or both, it is based on the type of the adsorbents used for development.

The basic principle of HPTLC:
Similar to other chromatographic processes such as column chromatography, HPLC, gas chromatography (GC), and paper chromatography, thin-layer chromatography is also based on the principle of separation. The separation of analytes is based on its relative affinity towards the stationary phase and mobile phase. Under the influence of the mobile phase, analytes are moves (by capillary action) over the surface of the stationary phase. The analytes have a higher affinity toward the stationary phase, they travel slowly while the others elute rapidly. When separation happens, the individual molecules are visualized as spots and we can determine the Rf value and other required parameters.

Applications of high-performance thin-layer chromatography (HPTLC):
  • Qualitative and quantitative analysis of compounds is the major application of high-performance thin-layer chromatography.
  • HPTLC used in the quality control of pharmaceuticals.
  • It is used to qualitative analysis of components in biological fluids and formulations.
  • For the detection of drugs and metabolites, it is used in pharmacokinetics.
  • The food industry uses HPTLC to estimate vitamins, nutrients, pesticides, and beverages from foodstuff, fruits, and vegetables.
  • High-performance thin-layer chromatography can use in many industrial applications
  • This method is significantly used in the forensic department for identification of substances, including drug abuse adulteration, and counterfeit drugs,
  • It can be used for the stability testing of substance.
  • High-performance thin-layer chromatography can use in environmental analysis.
Commonly asked questions on chromatography are as follows.
  

What is the major disadvantage between HPTLC

The major disadvantage of HPTLC is that it has a higher analysis cost than TLC.


What are the basic steps involved in the HPTLC procedure?

The basic steps involved in HPTLC are the selection of the stationary phase, selection and optimization of the mobile phase, preparation of the sample, application of sample, chromatogram development, and detection of the separated sample.


What is the principle of thin-layer chromatography (TLC)?

TLC operates on the principle that different molecules have different solubility and adsorption between two phases that separate them.


What is the major difference between HPTLC and TLC?

The major difference between HPTLC and TLC is the particle and pore size of the sorbents.


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