High-performance thin-layer chromatography (HPTLC) and thin-layer chromatography (TLC) both are planar chromatography methods that are used to separate the analytes on a flat thin layer of stationary phase using mobile phase. The HPTLC is an automated form of TLC. Several enhancements can be made to the conventional method of thin-layer chromatography to computerize the various steps, to increase the resolution obtained and automation is useful to overcome the errors caused by the manual process.
The major advantages of HPTLC over TLC includes,
The major advantages of HPTLC over TLC includes,
- Autosampler makes better than the TLC since it overcomes the manual error
- Provides high resolving power
- It reduces solvent consumption
- More rapidly development of chromatograms
- HPTLC has a broad range of stationary phase
- It utilizes UV/VIS or fluorescence detectors
- Enables simultaneous analysis of multiple compounds
- In HPTLC the sample spotting by the applicator
- It is highly précised than TLC
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