The thin-layer chromatography and column chromatography both are the types of chromatography that are used to separates the compounds. The basic principle of TLC and column chromatography is the adsorption of the solutes. The column chromatography and TLC are one of the most commonly used techniques in analytical techniques since they have many advantages and applications. Both techniques have the same stationary and mobile phases hence the TLC is used to find an appropriate mobile phase or solvent system and Rf value.
Here are mentioned some advantages of TLC over column chromatography.
Here are mentioned some advantages of TLC over column chromatography.
- The stationary phase of TLC is small in size compared to column chromatography, so a less sample amount is required.
- The Rf value in TLC can be easily calculated.
- TLC has a broad range of solvents than column chromatography.
- The results of thin-layer chromatography have more specific individual spots; therefore it becomes easier to differentiate them.
- TLC required fewer amounts of solvents to separate the analytes than the column chromatography.
- The molecules in TLC have to travel distance less as compared with column chromatography, hence it saves time.
- The preparation of samples in thin layer chromatography is easy as compared to column chromatography, the column chromatography required a large amount of sample.
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