Types of Ion-Pair Chromatography

Ion pair chromatography is an analytical technique used to separate charged molecules. It is broadly used to analyze acids and bases, especially with reverse phase chromatography (RP-HPLC). There are two types of ion-pair chromatography; these two types are as follows.
With conduct metric detector and chemical suppression:
A suppressor column next to after the analytical column is placed, this suppressor has high-capacity ion-exchanger and is the inverse of the analytical column, which converts eluent ions into slightly molecular species and does not retain the molecules ions.
With conduct metric detector and electronic suppression:
Mobile phases with very low and thin conductivity are used and its conductivity is corrected electronically.


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