Ion
pair chromatography is a technique for improving the separation of charged
analytes using the popular reversed-phase chromatography. The ion-pair reagents
are applied in the mobile phase to increase retention time and peak shape.
Here
some applications of ion-pair chromatography are mentioned.
Food
analysis:
It is
very useful for the analysis of food and beverages hence it is commonly used in
food industries. It can separate analytes with different chemical properties
such as polarity, acidity, and stability. As compared with ion-exchange
chromatography it gives better resolution and it can separate multiple vitamins
in a chromatogram.
Biological
analysis:
Ion
pair RP-HPLC is a versatile technique for RNA analysis; it can separate RNA
molecules.
Pharmaceutical
analysis:
Since
it is sensitive and can determine low concentrations of analytes, it can be used
in the pharmaceutical analysis to separate and determine impurities from the
dosage form.
Ion
pair chromatography has great accuracy and gives highly reproducible results.
It is a robust method as compared with RP-HPLC, as unaffected by changes in column
temperatures and pH of buffer or mobile phase.
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