Friday, November 22, 2019

Difference between normal and reversed-phase chromatography

High-performance liquid chromatography is a technique to separate the mixtures of sample components and is qualitative and quantitatively used in analytical chemistry. HPLC has different modes of separation such as reversed-phase chromatography, normal phase chromatography, affinity chromatography, and size exclusion chromatography, etc.

The main difference between normal phase chromatography and reversed-phase chromatography is that the NP-HPLC uses the polar mobile phase and a non-polar stationary phase while RP-HPLC uses a low polar mobile phase and a polar stationary phase.

The normal phase occurs when the mobile phase is non-polar while the stationary-phase that is sorbent is polar, which means that the more hydrophilic molecules the more it will interact with the stationary phase, hence its retention time increases inside the HPLC column.

The reversed-phase chromatography is mostly used the analytical technique to separate the analytes mobile phase is polar while the stationary phase is non-polar it means if the molecule is hydrophilic, it will have less interaction with the stationary phase results in polar analytes elutes quickly and non-polarity analytes elute slowly.
Posted by

No comments:

Post a Comment

Subscribe to: Post Comments (Atom)