Thursday, March 28, 2019

Advantages and Disadvantages of HPLC

High-performance liquid chromatography is one of the most popular and widely accepted separation techniques of chromatography, in which the mobile phase containing a sample of complex mixture pumped with a certain pressure through the column, which already filled with the adsorbent material, analytes interact with the adsorbent according to their properties and detector detects the retention time, area, and height of the peak. Compared to other technologies, HPLC has both advantages and disadvantages, let’s check it.
The advantage of HPLC:
  • HPLC offers a rapid, automated and highly precise method to recognize certain chemical components in a sample.
  • High-performance liquid chromatography offers a fast and precise quantitative analysis.
  • A gradient solvent system can be applied in certain methods.
  • It is highly reproducible.
  • HPLC can be upgraded to mass spectroscopy (MS).
  • The HPLC is very rapid, efficient, and delivers high resolution as compared to other chromatographic techniques, such as TLC, column chromatography, and paper chromatography.
  • Manages all areas of analysis to increase productivity
The disadvantage of HPLC:
  • HPLC can be an expensive method, it required a large number of expensive organics, needs a power supply, and regular maintenance is required.
  • It can be complicated to troubleshoot problems or develop new methods.
  • The lack of a universal detector for HPLC, however, the UV-Vis detector only detects chromophoric compounds.
  • The separation in High-performance liquid chromatography has less efficiency than GC.
  • It is more difficult for the beginner.
  • HPLC pump process reliability relies on of cleanliness of the sample, mobile phase, and proper operation of the system.

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