Column chromatography is a general technique that is used to separate the compounds from the complex mixtures. It can use small or large-scale column chromatography to separate and purify the analytes. Column chromatography has two phases, a mobile phase (liquid) and a stationary phase (solid). The sample mixture of compounds travels with the mobile phase through the stationary phase and basis of different degrees of adhesion it separates.The column chromatography works on the principle of adsorption. There are four different column chromatography types for several applications and that work on different mechanisms.
Advantages of column chromatography:
- Using column chromatography all kinds of complex mixtures can be separated.
- Any amount of mixture can be separated by column chromatography.
- A broad range of mobile phases.
- Analytes can be separated and reused, in preparative type chromatography.
- It can be possible to run automation.
- This is a robust method.
Disadvantages of column chromatography:
- It takes more time to separate the compounds.
- Column chromatography has of low separation power relative to advanced separation techniques.
- Higher quantities of solvents are essential, which is more expensive.
- Automation makes more complex and costly.
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