Tuesday, March 5, 2019

Applications of HPLC

HPLC is an analytical technique to separate, identify, and quantify components in a mixture. It is the single leading chromatography technique essential to most laboratories worldwide.
The use of HPLC can be done in both qualitative and quantitative applications, which are both for the identification and quantification of compounds. About all HPLC separation can be done in the reverse phase HPLC, Normal phase is not regularly used. The High-performance liquid chromatography can be applied in the pharmaceutical, drug science, molecular weight determination, environmental chemistry, in analytical chemistry forensic, food industries, and clinical tests.
Let’s check some applications of HPLC.
Pharmaceutical Applications of HPLC:
  • The tablet dissolution study of the pharmaceutical dosage form
  • To control the stability of the drug
  • The HPLC is used for drug quality control
  • The qualitative and quantitative study of analytes
  • It can be used to the mass determination by coupling it with mass spectroscopy (MS)

Applications of HPLC in Food and Flavour:
  • Analysis of preservatives
  • Analysis of sugar
  • Measuring soft drinks and water quality
  • Analysis of polycyclic
 Forensic Applications of HPLC:
  • It is used to determine the cocaine and other drugs of abuse
  • The quantity of drug in the biological samples
  • The HPLC is used for the identification of steroids in urine and blood
 Environmental Applications of HPLC:
  • The HPLC is used to finding phenolic compounds in drinking water
  • The HPLC is used for the identification and determination of compounds in drinking waters, air, waste, and the surface
  • It used for the determination of Bio-monitoring of pollutants.
Clinical Applications of HPLC:
  • Quantitative estimation hemoglobin
  • Analysis of urine
  • Analysis of antibiotics in blood
  • Analysis of Bilirubin
 
 
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