UV Spectroscopy is widely and basic used technique of analysis and used for the quantitative and qualitative study of analytes. UV is a fast medium of analysis and can give high accuracy, precision and it works on Beers- Lambert law. It is useful for various types of chemicals and components. Sample component absorbs the electromagnetic radiations of the ultraviolet and visible range and UV Visible spectroscopy measures these responses. Atoms and Molecules contain electronic transitions, whereas most solid substances contain inter bond transitions in the range of UV and visible.
Advantages of UV Visible Spectroscopy:
Advantages of UV Visible Spectroscopy:
- The core advantage is the accuracy of the UV-VIS spectrophotometer
- The UV-VIS spectrometer is easy to handling and use
- Provide robust operation
- UV-VIS spectroscopy is simple to operate
- Cost effective instrument
- Cover the entire of ultraviolet and visible
- It can be utilized in the qualitative and quantitative analysis
- The Derivative graph can be obtained by UV-VIS spectrophotometer
- It can be used in the degradation study of drug
- Only possible for the analytes which have a chromophore
- Only those molecules are analyzed which have chromophores
- The results of the absorption can be affected by pH, temperature, contaminants, and impurities.
- Only liquid samples are possible to analyze
- It takes time to get ready to use it
- Cuvette handling can affect the reading of the sample
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