High-performance thin layer chromatography (HPTLC) is a sophisticated, enhanced, and computerized form of thin layer chromatography. It is also known as flatbed chromatography, As compared to thin layer chromatography (TLC), it provides high-performance separations, there is different levels of operation at each step like Sample applications on HPTLC plates by auto applicants, selection of pre-coated stationary phases, Detecting compounds using different detection like UV, visible or fluorescence detectors and software used for data processing. All these benefits compared to TLC make HPTLC a better, high-performance and expensive technology.
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