Tuesday, October 16, 2018

What are the advantages of HPLC over TLC

High-performance liquid chromatography is the heir of thin-layer chromatography method and has contributed significantly to the analytical science development and its different applications in various fields like pharmaceuticals, clinical analysis, environmental analysis, polymers, and plastics. Each technique has its importance and utilities. It fulfills each other and all rely upon your necessities. Both techniques are used in the separation of drug products or analyte.
Here are mentioned some special highlights of HPLC, which makes the HPLC better than TLC, Let’s check some Advantages of HPLC over TLC.
Speed of analysis:
Mobile phase progression on a plate in TLC separation is dependent on capillary action or gravitational force. However, the flow of the mobile phase through the column in HPLC is speedy because it is pushed with the help of a pump.
Resolution:
TLC resolution makes it difficult to distinguish between overlapping spots and bands. On the contrary, in HPLC, it can be separated by controlling operational parameters such as mobile phase pH, mobile phase concentration, column temperature, and flow rate.
Sensitivity:
HPLC has a variety of detectors such as UV, photodiode array, mass, electrochemical, fluorescence, conductivity, and refractive index detectors, so it is a highly sensitive method. Such types of detectors are not available in the TLC analysis.
Quantification of results:
The HPLC programming is able to show exact and precise results based on the area of the peak since it’s suitable for quantitative analysis. But in TLC, Quantitation depends on the intensity matching of the spot which is not giving accurate and precise results.
Choice of stationary phase and columns:
Stationary phases are accessible in a large number, according to different categories of applications. Likewise, varieties of columns with different specifications are available in HPLC chromatography.
Reuse of Columns:
New TLC plates are required every time of TLC analysis, but in HPLC, columns can be reused for various components or complex blends.
Software:
HPLC is a computerized system, so it’s run on software and depends on peak area; it gives accurate and precise reports. It produces and saves the record of the chromatogram and all parameters, like mobile phase composition, flow rate, temperature, and wavelength, etc. are controlled through the software which is not possible in TLC analysis.

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