HPTLC is a widely accepted analytical technique and applies to a broad variety of analytes in various types of matrix. High-performance thin layer chromatography is one of the most broadly used methods for the pharmaceutical analysis, food and drug analysis, environmental analysis, cosmetic products, forensic chemistry, biochemistry, clinical chemistry, and other fields. It is the only chromatographic method which gives the option to present the result as an image.
Advantages of HPTLC (High-performance thin layer chromatography) include the following.
- Technically, learning and operating is easy.
- Many analysts work together on the system.
- Low Analysis Time and Low-cost Analysis.
- Low maintenance cost compared to other instruments.
- Visual recognition is possible because it is an open method.
- Availability huge ranges of stationary phases.
- UV-absorbing and corrosive mobile phases can be working on this system.
- Filtration and degassing for solvents are not necessary.
- Due to the use of the freshly prepared mobile phase and stationary phase, this does not have any chance of interference or contamination.
- Samples cleaning not often require.
- Sensitivity range of analysis usually occurs at the pictogram (pg) and Nanogram (ng).
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