Chrominfo: Applications of Thin Layer Chromatography in Pharmaceutical Analysis

Applications of thin layer chromatography in pharmaceutical analysis contain purity testing, identification, stability testing, assay, and content uniformity testing of intermediates, raw materials, and drug products, with the analysis of sample analytes.

TLC is dependent on its unique detection, the speed of analysis, flexibility, and simplicity methods on together quantitative and qualitative basis. Often degradation products, synthetic intermediates, and process related impurity they do not have chromophores which cannot be detected by the UV detector. Thus, these types of impurities are frequently specified by the TLC analysis. Sometimes impurities are eluted on the solvent front in the HPLC, and they may be complicated to quantities and monitor, and modify in the mobile phase or HPLC column could not be able to solve them adequately.

On the other hand, occasionally the impurities have taken more time to elute from the column, and they can’t be detected, but in TLC method is open in which whole samples are evaluated. Thin layer chromatography use in the early stage of drug development.

Here are some applications of thin layer chromatography mentioned. Let’s check it.

Separation of multi-component pharmaceutical formulations is one of the most significant applications of TLC.
TLC is also used to identify the components of traditional medicines.
Thin-layer chromatography is applied to identify the nature of various plant compounds.
The reaction mixture in the synthesis of chemical compounds can be detected by this method.
TLC can use for the identification of cations and anions in inorganic chemistry.
Preservatives, sweetening agents, colors, and various cosmetic ingredients are separated and identified using this technique.
TLC is a method for separating, analyzing, and characterizing metabolites and compounds in blood, urine, serum, and body fluids.
It is used in the food and cosmetic industry to identify drug residues.