HPTLC is TLC based analytical technique of separation; hence, adsorption is a principle of HPTLC, Where the solid stationary phase and the liquid mobile phase are used. The stationary phase is either coated on glass plates or aluminum foil of different sizes. If the plates are not freshly opened, they need to be activated before the application of the sample, using an Autosampler, a small amount (μl) of the sample or samples are applied to the bottom edge of the plate. By placing in a development chamber of HPTLC, this plate is developed, while the mobile phase travels up against gravity of the stationary phase up, separation of compounds due to the difference in relation to different compounds towards the stationary phase. The required time for the separation of the compound is called as development time, for the better separation, normally a distance of approximately 5 cm-15 cm is used. Separating spot can be seen using the densitometry scanner UV or visible or fluorescence detectors, it can be based on the properties of the components. Both qualitatively and quantitatively analysis can be done by using this method.
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