Tuesday, October 1, 2019

Factors Affecting Chromatography

Chromatography is an analytical technique generally used to separate mixtures of chemical substances into their individual- analytes with the help of the stationary phase and mobile phase. There are several kinds of chromatography e.g., Gas chromatography, liquid chromatography, affinity chromatography, ion-exchange chromatography, column chromatography, paper chromatography, thin-layer chromatography (TLC) however all of these works on the same basic principles.
Here are mentioned some factors affecting chromatography.
Factors affecting retention factor:
  • Type of stationary phase used
  • Eluent type
  • Analyte nature
  • Eluent composition
  • The solubility of the molecule in the solvent
Factors affecting efficiency:
  • Column length
  • The flow rate of the system
  • Particle size distribution
  • Particle diameter
Factors affecting chromatographic separation:
  • The mobile phase of the system
  • Composition of the mobile phase
  • Nature and composition of the stationary phase
  • Pressure of system
  • The temperature of the column
  • Length of the column
  • Total retention time (RT) of the system
  • The flow rate through a column
  • Structure, polarity, and size of the component
Factors affecting selectivity:
  • Nature of the component
  • Type of the stationary phase used
  • Temperature
  • Eluent additives
  • The composition of the mobile phase or solvents
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