Tuesday, July 23, 2019

What is the resolution in chromatography?

The adequate resolution and recovery of biological material is the objective for reversed-phase chromatography. The resolution, R, is usually defined as the distance between the centers of two peaks, as measured by the RT or volume divided by the average width of the peaks. Calculation of resolution is the easiest method for determining the actual separation obtained between two solute analytes. This relationship can be expanded to show the relationship between the resolution and chromatographic separation. Based on the isocratic elution the parameters have been derived from the chromatographic model, but it is still suitable when discussing the gradient elution and describing their effects on the resolution. Column selectivity, column retention factors, and column efficiency are the parameters that contribute to peak resolution.
Chromatographic peak resolution is given by the following equation.

Rs=2 tR2- tR1/ wb1+ wb2 
Where Rs is a resolution, tR is the retention time, and wb is the peak width at baseline.
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