Tuesday, July 23, 2019

What is dynamic binding capacity?

The binding capacity of a reversed-phase medium is quantitative confirms its the ability under static conditions to adsorb solute analytes or molecules. Dynamic binding capacity determines the available binding capacity on a particular flow rate. For the amount of analyte which will bind to a medium is corresponding to the concentration of immobilized ligand on the medium and furthermore relies upon the kind of molecule being adsorbed.
The dynamic binding capacity relies on the experimental conditions during binding, specific physical and chemical properties of the molecule, and reverses phase medium properties. The bead porosity is a significant factor which affects the binding capacity. The whole hydrophobic surface of macroporous media is accessible for the binding analyte. Large solute molecules can be expelled from small pore size media and just a small fraction of the entire hydrophobic surface will be used. When the highest binding capacity is necessary, a medium containing large pores should be used to permit all molecules of interest to be entered independently.
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