We know that the thin layer chromatography is a chromatographic method used to separate the components hence the selection of the appropriate mobile phase solvents is necessary to obtain optimal results of TLC. In fact, the vast strength of TLC lies in the broad range of applied mobile phase, which extends from polar to non-polar. An extremely polar mobile phase solvent is commonly used while the separation involves too many polar analytes, but a low polar mobile phase is suitable for components have low polarity. A very polar molecule is strongly attracted to the stationary phase (silica gel), thus a polar solvent is needed to move it.
Selecting a mobile phase for thin-layer chromatography:
Selecting a proper mobile phase system in chromatography is just a trial and error method. It is nothing that nothing is known regarding the solubility characteristics and chemical nature of a sample component. If we identify something regarding what the good solvent is for a compound, it may mean what the poor solvent would be. When we used good solvent as a carrier, the adsorbed sample will be move, while the poor solvent will probably carry nothing.Typically the primary step is to detect a mobile phase that will transfer the component with an Rf value 0.5. In complicated separations, a mobile phase may be required with changing composition. Interaction forces involved in TLC comprise inductive forces, charge transfer, intermolecular forces, covalent bonding, and hydrogen bonding. Thus, to know selectivity, it is necessary to know something about the nature of these different types of interactions.
Some examples of solvents used in TLC are ethanol, chloroform, water, hexane, benzene, ethyl acetate, and methanol, etc.
You may also like this
Effect of flow rate on column chromatography
Principle and Procedure of Thin Layer Chromatography
Effect of pH in reversed-phase chromatography
Effect of pH on HPLC analysis
Effect of pH on HPLC columns
Effects of Injection volume in HPLC
Factors affecting resolution in chromatography
Factors affecting column efficiency in column chromatography
Factors affecting column efficiency in HPLC
Theoretical plates in HPLC
HPLC column care and maintenance
Factors affecting reversed-phase chromatography
What is the capacity factor in chromatography?
Column efficiency in chromatography
What is the resolution in chromatography?
What is dynamic binding capacity?
Advantages and disadvantages of pH paper
No comments:
Post a Comment