The major difference between fluorescence and absorbance is that the absorption is the process that devours a photon and places a molecule or atom in an excited state. Fluorescence is the process that first devours a photon and places a molecule or atom into an excited state and after that emits photons that have low energy that takes the molecule or an atom reverse to the ground state.
The amount of light absorbed by a substance is measured in absorption spectrometer, while fluorescence spectrometry measures the intensity or the amount of light emitted by a molecule.
You may also like this
No comments:
Post a Comment