Fluorescence and absorption spectroscopy both are the most important laboratory techniques in chemistry. Both tools are sensitive, simple to operate, and can give a broad range of information. Both these methods are determined over the same region of wavelengths but are caused by two various phenomena. UV-Vis measures the absorption of the light near-ultraviolet region (200 to 400 nm) and the visible region (400 to 800 nm), whereas fluorescence spectroscopy measures the light emitted by a sample component in this region after absorbing light at an energy higher than that emitted.
The main difference between fluorescence spectroscopy and absorption spectroscopy is that the fluorescence spectroscopy measures the intensity or the amount of light emitted by an analyte, whereas absorption spectroscopy measures the amount of light absorbed by a particular component.
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