Chromatography is a method of physical separations that is applying for separate and recognizes analytes from the sample of complex mixtures. HPLC and TLC are the chromatographic techniques; both are useful for the separation of less volatile and non-volatile analytes. The important difference between HPLC and TLC is the quantitative separation of a sample is possible for HPLC, whereas TLC in which there is no quantitative separation of the sample.
Here are some points of difference between HPLC and TLC.
Here are some points of difference between HPLC and TLC.
- HPLC is a high-performance liquid chromatography.
- TLC is a thin layer chromatography.
- In HPLC, columns are used in place of plates and partition coefficient or adsorption is the method of separation.
- In TLC, the fine solid material is spread on a plate, and capillary action is permitted to migrate the mobile phase to the surface of the TLC plate.
- The liquid mobile phase in the HPLC system pumped with pressure through the HPLC column, which is packed with a stationary phase.
- The liquid mobile phase in TLC travels with capillary action through the TLC plate which is made of the silica or alumina.
- HPLC is high efficient than thin layer chromatography.
- TLC is less efficient than high-performance liquid chromatography.
- HPLC column is reusable.
- The TLC plate is not reusable.
- Very low sample quantity can be applied to TLC compared to HPLC.
- TLC takes less time for method development than HPLC.
- HPLC can be connected to Mass spectrometer.
- HPTLC is the automated form of TLC.
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