The
ultraviolet-visible spectroscopy (UV) is an important method of analytical
chemistry and it is one of the commonly used for the identification of
chemicals and qualitative analysis. However, quantitative analysis of various
inorganic and organic compounds is the most important use of UV. Principally,
UV spectroscopy is associated with the interaction of light with the matter
since, the light is absorbed by the substance/matter, the resultant energy
content of atoms or molecules increases. The absorption of ultraviolet or
visible light by an analyte will generate a specific spectrum, while
ultraviolet radiation is absorbed; it results in the electrons excitation in
the state of high energy from the ground state. The theory that revolves about
this concept suggests that the energy absorbed by ultraviolet radiation is
equivalent to the energy difference between the ground and high energy state.
UV
spectrophotometer principle adheres to the Beer-Lambert Law; this law expresses
that when a beam of monochromatic light is gone through a sample solution an
absorbing analyte. The diminishing rate of the radiation power alongside the
thickness of the sample solution is really corresponding to the incident
radiation and solution concentration.
Beer-Lambert
Law is expressed through this equation:
A
= log (I0/I) = ECI.
It
is clear from the Beer-Lambert law that the number of molecules able to absorb
the light of a specified wavelength, the light absorption limit is high. This
is the fundamental principle of UV spectroscopy.
You
may also like this
No comments:
Post a Comment