Preparation of dimethyl yellow indicator solution

Learn the procedure for making a dimethyl yellow indicator solution.

The compound that changes color when exposed to acidic or basic solutions is called an indicator. Most of the time, color indicators are used to measure pH. To find the endpoint or equivalence point of the titration, they are added to the reaction mixture.

Dimethyl yellow (C14H15N3) is an organic compound chemically described as 4-(Dimethylamino)azobenzene, N,N-Dimethyl-4-(phenylazo)aniline, Butter yellow, Methyl yellow. It is used as an acid-base indicator, as well as an indicator for non-aqueous titration which changes from yellow to red through a pH range pH 2.9 to pH 4.00.

Dimethyl yellow comes in the form of a yellow to orange-brown powder that is soluble in alcohol, benzene, chloroform, ether, and petroleum but, insoluble in water.

How to prepare dimethyl yellow indicator mixture for titration:

• Accurately weigh 100 mg of calcein and pour it into a 100 mL volumetric flask with 50 ml of rectified spirit.
• Once it is dissolved, dilute it to 100.00 mL with rectified spirit.
• The concentration of the prepared solution is about 01 g/L.

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Preparation of calcein indicator solution

Preparation of calcein indicator solution

Learn the procedure for making a calcein indicator solution.

The compound that changes color when exposed to acidic or basic solutions is called an indicator. Most of the time, color indicators are used to measure pH. To find the endpoint or equivalence point of the titration, they are added to the reaction mixture.

Calcein (C30H26N2O13), which is also called fluorexon or fluorescein complex, is a fluorescent dye with wavelengths of 495 and 515 nm for excitation and emission, respectively. It is used as a complexometric indicator for the titration of calcium ions with EDTA and fluorometric determination of calcium. 

Calcein is a fluorescent metal indicator, chemically described as 2, 7 Bis[bis(carboxymethyl)aminomethyl]fluorescein. Calcein comes in the form of a yellowish-orange powder that is slightly soluble in water.

In acidic conditions, a calcein solution emits a yellowish-green fluorescence, whereas there is no fluorescence in basic conditions. However, under basic conditions, Calcein will emit fluorescence in the presence of metal ions such as Al, Ba, Ca, Cu, Mg, and Zn.

How to prepare calcein indicator mixture for titration:

To make a fine powder that can be used as an indicator, mix 1 gram of calcein with 100 gm of potassium chloride.

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Preparation of dimethyl yellow indicator solution

Preparation of calcon indicator solution

Learn the procedure for making a calcon indicator solution.

The compound that changes color when exposed to acidic or basic solutions is called an indicator. Most of the time, color indicators are used to measure pH. To find the endpoint or equivalence point of the titration, they are added to the reaction mixture.

Calcon (C20H13N2NaO5S) is an azo dye chemically described as, 2-Hydroxy-1-(2-hydroxy-1-naphthylazo) naphthalene-4-sulfonic acid sodium salt that is used as an indicator (metal indicator) for complexometric titrations of calcium with ethylenediaminetetraacetic acid (EDTA) in the presence of magnesium.

Calcon comes in the form of a dark, blackish-brown powder that is soluble in water, ethanol, and methanol. It produces a purple color when calcium ions in an alkaline solution are present and a blue color when these ions are absent.

How to prepare calcon indicator for titration:

• Accurately weigh 100 mg of calcon and pour it into a 100 mL volumetric flask with 50 ml of water.
• Once it is dissolved, dilute it to 100.00 mL with water.
• The concentration of the prepared solution is about 01 g/L.

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Preparation of dimethyl yellow indicator solution