Learn the procedure for making dichlorofluorescein indicator solution.
The compound that changes color when exposed to acidic or basic solutions is called an indicator. Color indicators are commonly used to measure pH and are added to the reaction mixture to identify the titration endpoint or equivalence point.
Dichlorofluorescein (C20H10Cl2O5) is an organic dye of the fluorescein family, which is chemically described as 2′, 7′-Dichloro-3, 6-fluorandiol.
In Fajan’s method, it is used as an indicator for argentometric titration and as adsoprtion indicator. The color of the titration reaction changes from colorless to pale pink on reaching the equivalence point.
Dichlorofluorescein usually comes in the form of crystalline powder, in the color of orange to red-brown powder, which is soluble in ethanol and methanol but insoluble in water.
How to prepare dichlorofluorescein indicator for titration:
- Weigh 0.1 gm of dichlorofluorescein accurately and pour it into a 100.00 ml volumetric flask containing 50.00 ml of rectified spirit.
- Once it is dissolved, dilute it to 100.00 ml with rectified spirit.
- The concentration of the prepared solution is about 1.0 g/L.
References:
- Wikipedia contributors. (2022, June 30). Dichlorofluorescein. In Wikipedia, The Free Encyclopedia. Available Here:
- Law.resource.org. 2022. [online] Available Here:
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