Planar chromatography is a type of chromatography method used to separate a mixture of sample components, in which the stationary phase is on a flat plate and the mobile phase travel through capillary movement by stationary phase. There are two types of planar chromatography which are paper chromatography and thin-layer chromatography (TLC). Both techniques work on the same principles and methods, they employ different planes for the stationary phase.
The advantages of planar chromatography are as follows.
The advantages of planar chromatography are as follows.
- The analytes are separated by this method within a short period.
- A complex sample mixture can be easily separated as it is sensitive.
- Planar chromatography requires fewer sample volumes for analysis.
- This can be automated in the form of high-performance thin-layer chromatography HPTLC).
- Compared with other separation techniques it is an economical method.
- The use and setup of the planer chromatography are simple.
- This technique requires very few tools to carry out the process.
- In this method, organic as well as inorganic molecules can be possible to identify
- The use of the planar chromatography method cannot separate volatile components.
- Since it is an open system, humidity and temperature can affect the results.
- Another disadvantage of planar chromatography is that the molecules must be soluble.
- Planar chromatography isolates only small sample volumes. This has less accuracy compared with HPTLC and HPLC.
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