Sunday, December 29, 2019

Applications of LC-MS

The LC-MS technique uses an HPLC, in which the individual analytes in the sample mixture are first separated after ionization and the ions are separated based on their mass/charge ratio. The separated ions are after that directed through an electron multiplier tube detector, which recognizes each ion and determines its quantity. Mass spectrometry (MS) has played an essential role in many stages of the drug discovery process to identify and characterize lead molecules.
LCMS is useful in several industries for example biopharmaceutical, pharmaceuticals, food, forensic, and environmental sectors. Some applications of liquid chromatography-Mass spectrometry (LC-MS) are mentioned below.
Pharmaceuticals applications of LC-MS:
LC-MS is broadly used in the determination of pharmaceuticals and particularly in the separation of alternatively active drugs. Separation, detection, and purification of drug metabolites is one more major application of LC-MS. Separation and characterization of molecules in a sample mixture of natural products for example alkaloids, complex lipids, and fatty acids using LC-MS has been performed.
Quality Control:
Liquid chromatography-Mass spectrometry is a suitable technique for detection testing using a reference standard and for determining the purity of a drug substance or product.
Pharmacokinetic use of LC-MS:
Bioanalysis is commonly performed by an LC-MS / MS instrument since they have much higher sensitivity and identification as compared to UV detectors which are commonly used in high-performance liquid chromatography.
Environmental Applications of LC-MS:
This technique is employed in the analysis of various samples such as soil, sludge, air and drinking water or wastewater, etc.
Samples can belong to numerous different chemical classes, ranging from non-polar hydrocarbons to ionic organonomic classes.
Biomedical Applications of LC-MS:
The LC-MS method is functional for detecting steroids in body fluids. The high sensitivity detector using this method steroid sulfate can detect.


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