Wednesday, October 2, 2019

Effect of Column Length on Retention Time

Columns are available in different types and are used in different ways in different chromatography, such as high-performance liquid chromatography (HPLC), gas chromatography (GC), column chromatography, mass chromatography (MS), etc.
The column is an important factor in chromatography, as the column contains the separation of the compounds. It contains a stationary phase, from which the mobile phase passes through it and the separation of analytes happens at different retention times.
There are different types of factors that affect retention time. A change in column dimension leads to a change in the retention time of the compound, however, the amount of change varies for each peak.
Column dimensions will affect the sensitivity, efficiency, and analysis speed. The chromatographic column dimensions are selected according to the chromatographic application. Though, dimensions can likewise be modified to improve chromatographic peak by getting more sensitive, efficient, and rapid analysis. If the column is long, it will produce longer retention times but will have better separation. The only drawback of long columns is the high cost because it extends the run time. Short column lengths will provide shorter retention times and lower backpressures and are perfect for gradient analysis. 



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