Columns
are available in different types and are used in different ways in different
chromatography, such as high-performance liquid chromatography (HPLC), gas
chromatography (GC), column chromatography, mass chromatography (MS), etc.
The column is an important factor in chromatography, as the column contains the
separation of the compounds. It contains a stationary phase, from which the
mobile phase passes through it and the separation of analytes happens at different
retention times.
There
are different types of factors that affect retention time. A change in column
dimension leads to a change in the retention time of the compound, however, the
amount of change varies for each peak.
Column
dimensions will affect the sensitivity, efficiency, and analysis speed. The
chromatographic column dimensions are selected according to the chromatographic
application. Though, dimensions can likewise be modified to improve
chromatographic peak by getting more sensitive, efficient, and rapid analysis.
If the column is long, it will produce longer retention times but will have
better separation. The only drawback of long columns is the high cost because
it extends the run time. Short column lengths will provide shorter retention
times and lower backpressures and are perfect for gradient analysis.
You may also like this
No comments:
Post a Comment