Wednesday, September 11, 2019

What is the principle of colorimeter?


The colorimeter is a very sensitive instrument used to determine the concentration and intensity of a particular color used in a sample solution. There are generally two types of colorimeters that are used in industries that are spectrophotometer and tristimulus colorimeter. The principle of colorimeter is working on the base of Beer-Lambert's law. This law expresses that the absorption of light while goes through a medium is straightforwardly proportional to the medium convergence. While a colorimeter is used, there is a ray of light in which a particular wavelength is directed towards a sample solution. The beam of light passes through a series of different lenses before reaching a sample solution and the microprocessor is utilized for the calculation of the absorbance or transmittance of the light through the sample solution. If the absorption of the solution is high, there will be the high-light absorbed by the compound and if the sample solution has low concentration then more light will be transmitted through it.
Colorimetric reactions can be determined on a colorimeter or a spectrophotometer.
Both determine the light intensity that passing through a color sample compound and convert this intensity of the light to a concentration on the base of the stored standard calibration curve.
The colorimetry follows the principles of the Beer-Lambert Law is written as: 
A = Ɛ x b x c
A is the absorbance of the sample component
Ɛ is a wavelength-dependent absorptivity coefficient
b is the path length of the cell
c is the concentration of the analyte
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