Learn the identification tests of ferrous sulphate through a laboratory experiment or practical.
Aim:
To perform identification tests of ferrous sulphate on a given sample.
Reference: Indian Pharmacopoeia (IP) 2010
Requirements:
Glasswares and miscellaneous:
Test tube, test tube stand, test tube holder, measuring cylinder, pipette, glass rod, dropper, rubber stopper, volumetric flask, and balance, etc.
Chemicals:
Sulphuric acid (H2SO4), hydrochloric acid (HCL), 1,10-phenanthroline (C12H8N2), sodium hydroxide (NaOH), ceric ammonium sulphate ((NH4)4Ce(SO4)4), iodine (I2), potassium iodide (KI), stannous chloride (SnCl2), lead acetate (Pb(C2H3O2)2), barium chloride (BaCl2), ammonium acetate (C2H7NO2).
Preparation of chemicals:
Dilute H2So4 preparation:
Take 57.00 ml of concentrated H2So4of using a pipette and dilute to 1000.00 ml of distilled water in a volumetric flask, and properly mix it.
0.1% w/v 1,10-phenanthroline solution preparation:
Take 0.1 gm of 1,10-phenanthroline and dissolve to 100.00 ml of distilled water in a volumetric flask, and properly mix it.
0.1M ceric ammonium sulphate preparation:
In a mixture of 30.00 ml H2SO4 and 500 ml distilled water, dissolve 65.00 g ceric ammonium sulphate with the help of mild heat. Once cooling, dilute to 01 liters by distilled water and filter it. Filter the solution, if turbid,
Dilute HCL preparation:
Take 10.00 ml of concentrated hydrochloric acid (Approximately 10% w/w of HCL) using a pipette and dilute to 100.00 ml of distilled water in a volumetric flask, and properly mix it.
25 % w/v barium chloride solution preparation:
Take 25.00 gm of BaCl2 and dissolve to 100.00 ml with the same solvent in a volumetric flask, and properly mix it.
Iodine solution preparation:
Weigh accurately 02.00 gm of I2 and 03.00 gm of KI dissolve to 100.00 ml with the distilled water in a volumetric flask and properly mix it.
Stannous chloride solution preparation:
Accurately weigh 330.00 g of SnCl2 and pour in 100.00 ml of concentrated HCl, adding enough water to make 1000 ml.
Ammonium acetate solution preparation:
Weigh accurately 150.00 gm C2H7NO2 dissolve to 200.00 ml with the distilled water and add 03.00 ml of glacial acetic acid and add enough water to make 1000 ml. Make sure you are using a freshly prepared solution.
Procedure for identification of ferrous sulphate:
- Test 1: In 02.00 ml of water, dissolve a quantity of the substance under study containing 10 mg of iron. Then add 02.00 ml of dilute H2SO4 and 01.00 ml of a 0.1 % 1, 10-phenanthroline solution, an intense red color is formed, which discharged with adding a small amount of 0.1 M ceric ammonium sulphate is produced.
- Test 2: Take 50 mg of the sample and dissolve in 05.00 ml of water. Add 01.00 ml of dilute HCL and 01.00 ml of prepared BaCl2 solution; a white precipitate is formed.
- Test 3: Add 0.1 ml of iodine solution to the suspension produced in Test 2. The suspension stays yellow however is decolorized by dropwise adding prepared stannous chloride solution. Boil the mixture; no colored precipitate is formed.
- Test 4: Dissolved 20 mg of the sample substance in 02.00 ml of water and add 01.00 ml of prepared lead acetate, by which a white precipitate is formed that is soluble in ammonium acetate and sodium hydroxide solutions.
Observations:
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Identification Test |
Observation |
Inference |
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Test 1 |
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Test 2 |
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Test 3 |
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Test 4 |
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Results:
The given sample complies with the tests 1 / 2 / 3 / 4 and does not comply with the tests 1 / 2 / 3 / 4 for the identification of ferrous sulphate as per IP 2010.
It suggests that the given sample is of ………………………...
FAQ (Frequently Asked Questions):
What is the assay of ferrous sulphate?
The assay of ferrous sulphate is to find out the percentage purity, click here to get the procedure.
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