Thursday, May 3, 2018

Theoretical Plates (n) in chromatography

The theoretical plate number is a significant factor of the column; it is work as tool of column efficiency measurement. Its illustrate the quality of separation and ability to generate narrow and sharp peaks. Column having more number of theoretical pates are consider as more efficient in separation than the plates with less number, hence the peak resolution and narrow peak are depend upon the column efficiency.
N = 16(t/w)2
Hence
n = number of theoretical plates
t = retention time of analyte
w = width of the peak at base
The number of theoretical plates is reliant on various parameters like analyte characteristics, flow rate, sample size and temperature. As per the requirements theoretical plate number must be more than 2000.


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