Thursday, May 3, 2018

Resolution in Chromatography (Rs)

In chromatography analysis, if the sample having more than one compounds then need to be separate properly. If the retention time of two peaks have adequately different or peaks are sharp then get better resolution. The resolution shows the separation and sharpness of peak, an acceptable limit of resolution are Rs >1.5.
Rs = 2(t2-t1)/w1+w2
The equation of resolution shows t1 and t2 are retention time of first and second analyte, while w1 and w2 are bandwidth at the base.


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Retention Time (tR)
Capacity Factor (k')
Theoretical Plates (n)
Dead Volume (Vo)
pKa Value
Mobile Phase of HPLC

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