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Saturday, May 2, 2020

What are the uses of chromatography?

Chromatography is the method used to isolate compounds or a sample mixture according to the relative affinity of each compound distributed amongst a stationary phase and a mobile phase. The compounds can be separated based on the different processes and the presence of characteristics, for example, hydrophobic groups present on the surface, size and shape, binding capacity with the stationary phase, and total charge. There are four main types of chromatography techniques are available such as partition chromatography, ion-exchange chromatography, adsorption chromatography, and size exclusion chromatography. Other chromatography methods are also available based on the stationary bed, such as high-performance liquid chromatography (HPLC), column chromatography, thin-layer chromatography (TLC), gas chromatography (GC), and paper chromatography, etc.
The chromatography has many applications in various fields which are mentioned below.
  • The chromatography is used in food processing for quality control.
  • Chromatography is used to check air quality and also the analysis of water samples.
  • It can use to determine the number of organic acids in foods that give significant information about food quality.
  • Chromatography is also used to determine the amount of drug present in blood samples, or urine.
  • It is used for the detection of contaminants (pesticides) or heavy metal in foods and food water.
  • It is mainly used in pharmaceuticals to verify the quality of the product or drug substance.
  • Chromatography is used in qualitative and quantitative analysis of molecules.
  • It is used to isolate solutions of dyes and pigments.
  • It is widely used in the forensic department to determine the alcohol or drug abuse.
  • Chromatography is also used in environmental applications, clinical diagnosis of diseases, petroleum industries, cosmetics, and herbal products, etc.

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