What is ion-pairing reagent and why is used in HPLC

The ion-pairing reagent is generally an alkyl sulfate, an alkyl sulfonate, or an alkylammonium salt. Ion pairing reagents consist of ionic molecules, which have a charge opposite to component of interest as well as a sufficiently the hydrophobic region, which permits the stationary phase, which interacts with the associated counter-ions.

• Why do we need an ion-pair HPLC?

The ion-pairing reagents or ion pair chromatography is used while a sample has ionic molecules that are very hydrophilic, and therefore reversed-phase retention can be problematic. It’s also used when other modifications in reversed-phase chromatography (RP-HPLC) conditions did not succeed to achieve adequate resolution.

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