The capacity factor (k´) is associated with the retention time (tR) and is an indication of the proportion of time of any particular analyte in the stationary phase contrary to the mobile phase. The long retention times the consequence in large values of capacity factor.
The capacity factor can be calculated for every peak recognized in a chromatogram by using the following equations. Capacity Factor=K´=M0les of solute in the stationary phase / Moles of solute in the mobile phase
K´= TR–To/To VR-Vo/To
Where TR is the retention time VR is the retention volume of the molecule, and To and Vo the retention time and retention volume, respectively of an unretarded solute. The capacity factor means an indication that how long an analyte can be retained by a stationary phase.
The capacity factor can be calculated for every peak recognized in a chromatogram by using the following equations. Capacity Factor=K´=M0les of solute in the stationary phase / Moles of solute in the mobile phase
K´= TR–To/To VR-Vo/To
Where TR is the retention time VR is the retention volume of the molecule, and To and Vo the retention time and retention volume, respectively of an unretarded solute. The capacity factor means an indication that how long an analyte can be retained by a stationary phase.
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