UV-Visible Spectrophotometer is one of the mainly used techniques in drug analysis. This technique used to detect the concentration of absorbed UV-visible radiation by the analyte. In the UV-VIS spectroscopy, the ratio of proportion, the ratio of the two beams intensity of light is called an ultraviolet-visible spectrophotometer. In the qualitative analysis of UV-Vis, the organic compounds can be identified using a spectrophotometer, the quantitative spectrophotometric analysis is used to detect the amount of radiation absorbed by the compound. The spectrophotometric technique is fast, simple, modest, and applies to small amounts of compounds. Beer-Lambert's law is the law that regulates quantitative spectrophotometry analysis.
Beer’s law:
It is in Simple words the absorption is proportional to concentration; this law shows the light intensity of the monochromatic radiation diminishes quickly with the number of molecules absorbed.
Lambert’s law:
This law shows the intensity of the beam of parallel monochromatic radiation diminishes quickly as it goes through the uniform thickness.
A combination of these two laws generates Beer-Lambert law.
The region of UV -Visible and infrared.
Region Wavelength (Range)
Far-ultraviolet 10-200 nm
Near ultraviolet 200-400 nm
Visible 400-750 nm
Near-infrared 0.75- 2.2 μm
Mid-infrared 2.5-50 μm
Far infrared 50-1000 μm
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