High-performance liquid chromatography (HPLC) is a widely used separation technique for the qualitative and quantitative analysis of compounds. HPLC, also known as high-pressure liquid chromatography, it is an advanced version of LC. In HPLC, we inject the sample solution through the syringe into the injector, the mobile phase carries it through a stationary phase (column) that isolates the molecule based on its affinity.HPLC has a wide range of applications, such as pharmaceuticals, forensics, clinical, and food analysis.
The major difference between traditional LC and HPLC is that the mobile phase of liquid chromatography moves by the gravity force while the mobile phase of HPLC flows through the high pressure that is created by the pump, which decreases the analysis time.
The major difference between traditional LC and HPLC is that the mobile phase of liquid chromatography moves by the gravity force while the mobile phase of HPLC flows through the high pressure that is created by the pump, which decreases the analysis time.
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