Chromatography is an analytical technique generally used to separate mixtures of chemical substances into their individual- analytes with the help of the stationary phase and mobile phase. There are several kinds of chromatography e.g., Gas chromatography, liquid chromatography, affinity chromatography, ion-exchange chromatography, column chromatography, paper chromatography, thin-layer chromatography (TLC) however all of these works on the same basic principles.
Here are mentioned some factors affecting chromatography.
Factors affecting retention factor:Here are mentioned some factors affecting chromatography.
- Type of stationary phase used
- Eluent type
- Analyte nature
- Eluent composition
- The solubility of the molecule in the solvent
- Column length
- The flow rate of the system
- Particle size distribution
- Particle diameter
- The mobile phase of the system
- Composition of the mobile phase
- Nature and composition of the stationary phase
- Pressure of system
- The temperature of the column
- Length of the column
- Total retention time (RT) of the system
- The flow rate through a column
- Structure, polarity, and size of the component
- Nature of the component
- Type of the stationary phase used
- Temperature
- Eluent additives
- The composition of the mobile phase or solvents
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