Advantages and Disadvantages of Affinity Chromatography

Affinity chromatography in which a macromolecule, (for example, a protein) is separated and purified in a solution with a molecule containing a ligand for which the macromolecule has an affinity that reason it is retained on the column.

The Advantages of Affinity Chromatography are:

• High sensitivity compared to TCD & FID.
• Affinity chromatography is used in the production of vaccines.
• Affinity chromatography is used in the purification of protein and enzyme.
• Affinity chromatography gives High specificity.
• Enzymes and other proteins are studied by affinity chromatography.
• To maintain the quality of the product, this chromatography is used in the pharmaceutical manufacturer in the production of vaccines.
• Affinity chromatography doesn’t rely on ionic strength, pH, temperature, and composition of the buffer.
• The high degree of purity can be obtained by Affinity Chromatography.
• This is a very reproducible process.
• This is the simplification method.
• Used to increase the solubility.
• In genetic engineering, affinity chromatography is used.

The Disadvantages of Affinity Chromatography are:

• It takes a lot of skill to handle it.
• It interferes with the structure.
• Transfer and the leakage of metal ion lead to protein loss.
• Sometimes ligands leakage is observed.
• The volume of the sample is limited.
• The carrier gas used must be pure such as pure nitrogen.
• The ligands used in affinity chromatography are costly.
• Relatively low productivity.
• It has a non-specific adsorption.
• Degradation of the solid support.
• Metal-ion transfer and metal ion leakage lead to loss of protein.
• Affinity chromatography is non-specific for adsorption than other chromatography methods.

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