Chrominfo: Applications of UV Visible Spectroscopy

An analysis by the spectrophotometric methods attains accurate and fast results with small quantities of sample. This is the most efficient tool for its compatibility and economic value in pharmaceutical analysis. Qualitative analysis has confirmed to be extremely useful in many forms of organic compounds.
Some applications of UV Visible spectroscopy are listed below.
As an HPLC detector:

Most analytes can be analyzed by UV / visible detectors and this UV detector is used in HPLC, hence the UV detector makes the most widely used and useful LC detector.

Quantitative Analysis:

Compounds that absorb UV radiation can be used for quantitative determination based on Beer’s law to find out the unknown concentration of a sample component by the absorption spectrometry. Most organic compounds are absorbed in the range of the UV-visible spectrum.

Qualitative Analysis:

Visible and UV spectrophotometer can be utilized to identify compounds through by plotting the spectrum curve and compared with the known spectrum.

PKa value can be calculated:

Spectrophotometrically PKa value can be determined from the graph plotted at various pH values between the wavelength and absorption.

Detection of Impurities:

To determine impurities in organic molecules, UV absorption spectroscopy is a better way. Due to impurities in the sample, additional peaks can be seen and compared to standard materials. By measuring absorption at particular wavelengths, impurities can be detected

Measuring reaction rates:

The rate of reaction can also be analyzed using UV spectroscopy. By observing absorbance changes of the sample throughout the reaction the rate of reaction can be measured.

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