The adsorption chromatography is a form of chromatography and it is the most widely used method in the form of column chromatography, gas-solid chromatography, and thin-layer chromatography. Adsorption chromatography consists of a stationary phase (fine solid partitioned) and a mobile phase (solvent or a mixture of solvents). The analyte differently interacts with the stationary phase because every analyte has a different affinity towards the adsorbent. Therefore, the separation of molecules occurs at different retention times.
The advantages of adsorption chromatography are as follows.
The advantages of adsorption chromatography are as follows.
- It has a wide range of mobile phases for the separation of compounds.
- Adsorption chromatography is an important method to separate many components that are not separated by other techniques.
- The complex sample mixtures can be easily separated by this method.
- Very few types of apparatus or types of equipment are required for isolation.
- Obtained results from some methods of adsorption chromatography are complex to reproduce.
- This can cause catalytic variations.
- Some solutes take retention time to separate this is a major disadvantage of adsorption chromatography.
- It is more complicated and costly when make automation.
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