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Saturday, April 4, 2020

Advantages and disadvantages of ion-exchange chromatography

Ion-exchange chromatography is a type of HPLC chromatography and is used for purification of proteins and other charged molecules. The molecules are separated in this method, depending on their charge. In this type of chromatography, the negatively charged analytes are attracted with a positive charge to solid supports. Conversely, in cation-exchange chromatography positive charged analytes are pulled into a negatively charged solid support. Cation exchange chromatography and anion exchange chromatography are two types of ion-exchange chromatography
The advantages of ion-exchange chromatography are as follows.
  • Ion-exchange chromatography is one of the most powerful methods of separating charged particles.
  • Using this method the inorganic ions can also be separated.
  • It can use more commonly for both analytical and preparative purposes.
  • Almost all charged molecules such as small amino acids, nucleotides and large proteins can separate using this method.
  • This is very effective and powerful water softening method.
  • Resins have a long life.
  • It has cheap maintenance.
The disadvantages of ion-exchange chromatography are as follows.
  • The buffer requirement is the major disadvantage of ion-exchange chromatography.
  • It has a high working cost since the buffer is used for the separation of components.
  • This method can only be used to isolate charged molecules.
  • Sodium ions entering the soft water will increase the acidity level in the water.

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