Advantages of fluorescence spectroscopy over UV

Absorption and fluorescence are different, but integral techniques of quantitation of molecules. Fluorescence spectroscopy has numerous advantages over ultraviolet-visible spectroscopy. It’s very low detection limit is a major advantage of fluorescence spectroscopy over ultraviolet-visible spectroscopy. It can be a highly sensitive than the absorption measurement in the ultraviolet spectrophotometer.
The advantages of fluorescence spectroscopy over UV are as follows.
High Sensitivity: Because of the high extinction coefficient of fluorophores, fluorescence assay is very sensitive and it allows the molecule detection at hundreds of times lower concentrations than those detectable by conventional absorption.
Specificity: The binding properties of fluorophores make this technique extremely selective for particular components.



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