Definition of partition column chromatography

Column partition chromatography is not widely used technology like column chromatography, but all techniques and requirements are similar to column chromatography. This chromatography technique is parallel to the adsorption column chromatography, excluding that the stationary phase is liquid. The silica gel or cellulose is used as a solid support to grasp the stationary phase (liquid) like aqueous buffer solutions or water as on the surface like a thin film. In this type of chromatography mobile phase is parallel to the column chromatography; however, in this technique gradient elution is not used, since the equilibrium will be upset.
Column chromatography is used to isolate solids and liquids from the mixture of the sample solution. There are many applications of column chromatography, one of the major applications is to separate analytes into individual components. The adsorption is the basic principle of column chromatography in which the solutes are separated by using its polarity. Different types of column chromatography are used for separation such as partition chromatography, ion-exchange chromatography, gel chromatography, and adsorption chromatography. This classification of column chromatography based on the mechanism of separation.


You may also like this

What is Column Chromatography
Pharmaceutical Applications of Column Chromatography
Factors Affecting Separation in Column Chromatography
Advantages and Disadvantages of Column Chromatography
Different types of Column Chromatography
Difference between gas chromatography and liquid chromatography
What are the Advantages of HPLC over GC
Advantages and disadvantages of paper chromatography
Advantages of TLC over Paper Chromatography