Learn the procedure for making phenosafranine indicator solution.
The compound that changes color when exposed to acidic or basic solutions is called an indicator. Color indicators are commonly used to measure pH and are added to the reaction mixture to identify the titration endpoint/equivalence point.
Phenosafranine (C18H15ClN4) is a compound or dye, chemically described as 3,7-Diamino-5-phenylphenazinium chloride.
Phenosafranine is a phenylphenazinium bacterial stain for microscopy that is used as an indicator in adsorption and redox titration.
It turns the precipitate red when used in the titration of chloride or bromide with silver nitrate in an acid solution. Once the precipitate is complete, the color of the precipitate changes to blue color.
Phenosafranine usually comes in the form of powder, crystals, or flakes, and its appearance of dark green powder which is soluble in water.
How to prepare phenosafranine indicator for titration:
- Weigh accurately 0.25 gm of phenosafranine and pour it into a 100.00 ml volumetric flask containing 50.00 ml of distilled water and mix it.
- Once it is dissolved, dilute it to 100.00 ml with distilled water.
- The concentration of the prepared solution is about 2.5 g/L.
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