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Saturday, April 4, 2020

Advantages and disadvantages of planar chromatography

Planar chromatography is a type of chromatography method used to separate a mixture of sample components, in which the stationary phase is on a flat plate and the mobile phase travel through capillary movement by stationary phase. There are two types of planar chromatography which are paper chromatography and thin-layer chromatography (TLC). Both techniques work on the same principles and methods, they employ different planes for the stationary phase.
The advantages of planar chromatography are as follows.
  • The analytes are separated by this method within a short period.
  • A complex sample mixture can be easily separated as it is sensitive.
  • Planar chromatography requires fewer sample volumes for analysis.
  • This can be automated in the form of high-performance thin-layer chromatography HPTLC).
  • Compared with other separation techniques it is an economical method.
  • The use and setup of the planer chromatography are simple.
  • This technique requires very few tools to carry out the process.
  • In this method, organic as well as inorganic molecules can be possible to identify
The disadvantages of planar chromatography are as follows.
  • The use of the planar chromatography method cannot separate volatile components.
  • Since it is an open system, humidity and temperature can affect the results.
  • Another disadvantage of planar chromatography is that the molecules must be soluble.
  • Planar chromatography isolates only small sample volumes. This has less accuracy compared with HPTLC and HPLC.

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