The
LC-MS technique uses an HPLC, in which the individual analytes in the sample
mixture are first separated after ionization and the ions are separated based
on their mass/charge ratio. The separated ions are after that directed through
an electron multiplier tube detector, which recognizes each ion and determines
its quantity. Mass spectrometry (MS) has played an essential role in many
stages of the drug discovery process to identify and characterize lead
molecules.
LCMS is
useful in several industries for example biopharmaceutical, pharmaceuticals,
food, forensic, and environmental sectors. Some applications of liquid
chromatography-Mass spectrometry (LC-MS) are mentioned below.
Pharmaceuticals
applications of LC-MS:
LC-MS
is broadly used in the determination of pharmaceuticals and particularly in the
separation of alternatively active drugs. Separation, detection, and
purification of drug metabolites is one more major application of LC-MS.
Separation and characterization of molecules in a sample mixture of natural
products for example alkaloids, complex lipids, and fatty acids using LC-MS has
been performed.
Quality
Control:
Liquid
chromatography-Mass spectrometry is a suitable technique for detection testing
using a reference standard and for determining the purity of a drug substance
or product.
Pharmacokinetic
use of LC-MS:
Bioanalysis
is commonly performed by an LC-MS / MS instrument since they have much higher
sensitivity and identification as compared to UV detectors which are commonly
used in high-performance liquid chromatography.
Environmental
Applications of LC-MS:
This
technique is employed in the analysis of various samples such as soil, sludge,
air and drinking water or wastewater, etc.
Samples
can belong to numerous different chemical classes, ranging from non-polar
hydrocarbons to ionic organonomic classes.
Biomedical
Applications of LC-MS:
The
LC-MS method is functional for detecting steroids in body fluids. The high
sensitivity detector using this method steroid sulfate can detect.
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Difference between HPLC and LCMS
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Difference between HPLC and LCMS
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