Affinity chromatography
is the method by which the interactions of reversible biospecific between
molecular species, its legand is utilized in the separation of such molecular
species from a biological environment. Affinity is used to tell how two
molecules behave with one another. Polar substances have a preference for each
other to the substance of non-polar and we generate antibodies with intimacy to
antigen for help fight against the infections. Affinity chromatography depends
on biological relationships between molecules such as substrates and enzymes
there is an affinity for each other. As the method depends on this molecule
interaction, it could be separate a molecule based on its individual chemical
composition and it is the only method that can execute this.
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