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Sunday, April 12, 2020

Advantages and disadvantages of fluorometry

Fluorometry is an analytical device for fluorescence determination and measurement in compounds that use ultraviolet light in compounds. It is an analytical method for detecting and measuring fluorescence in molecules that use fluorescence light in a molecule. Fluorometry technique is mostly applicable to low concentration ranges and is consequently sensitive analytical methods compared to spectrophotometric determination. Typically, fluorometric methods have sensitivities that are two to four orders of magnitude and higher compared to spectrophotometric procedures.
The advantages of fluorometry are as follows.
  • The major advantage of fluorometry is its high sensitivity compared to other absorption methods.
  • Fluorometry has a high specificity due to unique optical properties compounds.
  • This will measure the intensity of fluorescence, and sample concentration.
  • It is more precise on the characteristics of wavelengths of excitation and emission.
  • It is more precise than the absorption process because both emission and excitation wavelength are characteristics.
  • Fluorometry has a wide range of concentrations.
  • It is a user-friendly and economical method.
The disadvantages of fluorometry are as follows.
  • The major disadvantage of the fluorescence method is that it only analyzed fluorescent molecules, not all.
  • Rapid scanning is not possible to obtain the excitation and emission spectrum of the analyte.
  • Reference and sample solution cannot be analyses at a time.
  • It is not useful in identification.
  • The dilute sample solutions of compounds are less stable.

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